The enzyme is a taq dna polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. Certificate of analysis gotaq hot start polymerase. Pdf reverse transcriptase inhibits taq dna polymerase. Polymerase chain reaction, 122004 2 materials template dna genomic, plasmid, bacterial colony, etc. The 5x green gotaq flexi buffer contains two dyes a blue dye and a yellow dye that separate during electrophoresis to. The 5x green gotaq reaction buffer contains two dyes blue and yellow that. Promega pcr product we will send a replacement or refund your account. Gotaqr flexi dna polymerase product information sheet 9pim829.
Taq dna polymerase is the industry standard for routine pcr. The master mix contains everything needed to perform long pcroptimized buffer, dntps, mgcl 2 and an optimized hotstart enzyme blend for long pcr. Gotaq long pcr master mix a is a readytouse solution for long pcr targets. Dna polymerase and taq dna polymerases from other vendors b promega gotaq g2 dna polymerase, c bioline mytaq dna polymerase, d neb onetaq dna polymerase, e takara taq dna polymerase, and f kapa biosystems taq pcr kit according to manufacturers recommendations.
Onetaq dna polymerase is an optimized blend of taq and deep vent dna polymerases for use with routine and difficult pcr experiments. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. This lowtemperature polymerase activity is the basis for nonspecific amplification associated with mispriming events that occur during the initial phase of. Taq dna polymerase is a thermostable enzyme that synthesizes dna from singlestranded templates in the presence of dntps and a primer. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified dna from the pcr. Efficiency comparison of seven different taq polymerases. Dreamtaq hot start green dna polymerase 200 u 500 u 2,500 u 4 x 2,500 u ep1711 ep1712. Expand high fidelity pcr system is composed of a special enzyme mix containing thermostable taq dna polymerase and tgo dna polymerase, a thermostable dna polymerase with proofreading activity. Efficiency comparison of seven different taq polymerases used in hemogenetics j. A unique 10x reaction buffer for use with taq dna polymerase in reaction systems which require a detergent free. Pcr amplification an introduction to pcr methods promega. Promega go taq master mixes gotaq green master mix.
Reverse transcriptase inhibits taq dna polymerase activity article pdf available in nucleic acids research 207. The gene has been cloned and used to produce the enzyme in nonthermophilic host bacteria so both native taq, isolated from thermus aquaticus, and cloned taq, isolated from expression systems in other bacteria, are commercially. Gotaq dna polymerase a,b is a taq dna polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. Currently, str typing is the most efficient and the fastest way for identification of. Pcr kit, b neb onetaq hot start dna polymerase, c promega gotaq g2 dna polymerase, d toyobo quick taq hs dyemix, e roche faststart taq dna polymerase, and f sigmaaldrich jumpstart taq dna polymerase. There are three main patents relating to the pcr process and taq dna polymerase. Cycling times for each polymerase are shown in purple, while ramping times on the proflex pcr system.
The 5x green gotaq reaction buffer contains two dyes blue and yellow that separate during electrophoresis to monitor migration progress. Purification of taq dna polymerase for 1 liter culture modified from the protocol presented in f. Visit our product page for additional information and protocols. Gotaqr qpcr master mix technical manual tm318 promega. Neb provides high quality recombinant taq at an exceptional value. Taq polymerase for robust pcr with and directtogel convenience.
Gotaq green master mixa,b is a premixed readytouse solution containing bacterially derived taq dna polymerase, dntps, mgcl2 and reaction. Commercial producers have sinced cloned and expressed the enzyme in e. Taq is available with different formats to accommodate a variety of pcr applications. The enzyme consists of a single polypeptide with a molecular weight of 94 kda.
Janica b a central forensic laboratory of the police, warsaw, poland b department of forensic medicine, medical university of bialystok, poland abstract. White paper platinum ii taq hotstart dna polymerase platinum ii taq hotstart dna polymerase for highthroughput pcr platinum ii taq hotstart dna polymerase is an innovative pcr reagent designed to address these challenges. Gotaq dna polymerase product information promega corporation. The following file naming structure is used to name the majority of these document files. Phusion dna polymerases also produce higher yields while using less enzyme than traditional proofreading polymerase reactions. Gotaq g2 dna polymerase reliably amplifies a wide range of pcr templates and. The onetaq reaction buffers and high gc enhancer have been formulated for robust yields with minimal. Comes with qiagen hotstartaq dna polymerase kit mix equal volumes of 100 mm stocks of invitrogen datp, dttp, dctp and dgtp primer stocks are at 100 um. The system contains a fluorescent the system contains a fluorescent dnabinding dye, the bryt green dye, that exhibits greater fluorescence enhancement upon binding to double. Taq dna polymerase and taq pcr core kit en print bookmark share pdf 63kb english format file size language download get adobe reader contact qiagen. The ptactaq vector, carrying the taq pol coding region was a kind gift of dr. Add 95 ul water for every 5 ul primer to obtain 5 um solution.
Toll free in the usa 8003569526 telephone 6082744330 internet. This powerful polymerase mixture is designed to generate pcr products of high yield, high fidelity and high specificity from all types of dna 1. The supplied 5x green and colorless flexi reaction buffers do not contain magnesium. This polymerase is an enginereered taq enzyme capable of. Promega corporation 2800 woods hollow roadmadison, wi 537115399 u. Use of taq polymerase as model system is a good way to introduce ip patent system, licensing issues, how patents are structured, and recent changes to patent system. Gotaq qpcr master mixa,b is a reagent system for quantitative pcr qpcr. Production et purification rapide dune taq polymerase pour les. This high processivity results in shorter extension times, more robust amplification and the ability to amplify long templates up to 20 kb in a fraction of the time.
Only the template and primers need to be added to amplify your target. The thermostable thermus aquaticusdna polymerase taq pol has been the key factor in transforming the initial pcr method into one with huge impact in molecular biology and biotechnolo. Polymerase chain reaction pcr is a rapid procedure for in vitro enzymatic amplification of specific dna sequences using two oligonucleotide primers that hybridize to opposite strands and flank. Gotaqr pcr core system technical bulletin tb254 promega. Hot start taq dna polymerase is a mixture of taq dna polymerase and an aptamerbased inhibitor. Gotaq green master mix product information promega corporation. Primers resuspended in sterile water or te to a concentration of 100 mm buffer usually 10x, usually sold with taq polymerase or you can make your own. Promega go taq master mixes prm7123 print share promega go taq master mixes. It is supplied with 10x standard taq reaction buffer, which is detergentfree and designed to be compatible with existing assay systems. All our enzymes are tested extensively for the absence of nicking and priming activities, exonucleases. Since it was first isolated, taq dna polymerase has become the standard reagent for the pcr reaction. Because taq dna polymerase is a nonproofreading polymerase, pcr products generated with taq dna polymerase will contain a singlenucleotide 3.
Day 1 start 3ml overnight culture of taq from glycerol stock in lbamp 75ngul day 2 add 1ml of overnight culture to 1l of lbamp. Enhanced performance over standard taq dna polymerase. Like standard taq, it has both 5 to 3 polymerase and 5 to 3 exonuclease activity, but lacks 3 to 5 exonuclease activity. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. High performance dna polymerases are purified according to multiple purification protocols minimizing the amount of e. New formulation of taq dna polymerase with enhanced performance over standard taq dna polymerase. Pluthero 1993 rapid purification of highactivity taq dna polymerase. First isolated from the bacterial thermophile thermus aquaticus, the taq polymerase has become the staple enzyme in molecular biology. Gotaq dna polymerase m300 protocolpdf 207 kb english. Thermo scientific generuler 1 kb plus dna ladder was used. The green master mix allows direct gel analysis after amplification. Gotaq g2 dna polymerase product informationpdf 191 kb english.
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